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Title: A pilot analytic study of a research-level, lower-cost human papillomavirus 16, 18, and 45 test.
Authors: Yang HP,  Walmer DK,  Merisier D,  Gage JC,  Bell L,  Rangwala S,  Shrestha N,  Kobayashi L,  Eder PS,  Castle PE
Journal: J Virol Methods
Date: 2011 Sep
Branches: CGB, HREB
PubMed ID: 21640138
PMC ID: PMC3155880
Abstract: The analytic performance of a low-cost, research-stage DNA test for the most carcinogenic human papillomavirus (HPV) genotypes (HPV16, HPV18, and HPV45) in aggregate was evaluated among carcinogenic HPV-positive women, which might be used to decide who needs immediate colposcopy in low-resource settings ("triage test"). We found that HPV16/18/45 test agreed well with two DNA tests, a GP5+/6+ genotyping assay (Kappa = 0.77) and a quantitative PCR assay (at a cutpoint of 5000 viral copies) (Kappa = 0.87). DNA sequencing on a subset of 16 HPV16/18/45 positive and 16 HPV16/18/45 negative verified the analytic specificity of the research test. It is concluded that the HPV16/18/45 assay is a promising triage test with a minimum detection of approximately 5000 viral copies, the clinically relevant threshold.